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西安交通大学仿生工程与力学研究所(BEBC)研究人员**通过HEKA ELP3扫描电化学显微镜揭示细胞力学微环境对心肌细胞氧化还原水平的调控作用

日期:2020-09-04 22:19
浏览次数:492
摘要:西安交通大学仿生工程与力学研究所(BEBC)研究人员**通过HEKA ELP3扫描电化学显微镜揭示细胞力学微环境对心肌细胞氧化还原水平的调控作用。该成果以“Effect of substrate stiffness on redox state of single cardiomyocyte: a scanning electrochemical microscopy study”为题发表在Analytical Chemistry上。

XINJIZUZHIZHONGXINJIXIBAOCHUYUFUZAQIECHIXUBIANHUADELIXUEWEIHUANJINGZHONG,QIZHONGJIZHIYINGDUBEIZHENGSHIKEDIAOKONGXINJIXIBAODEBIAOXINGHEGONGNENG,JINERYINGXIANGXINJIXIBAODECHENGSHUXINGHUOYINQIRUXINJIFEIHOUDENGBINGLIGUOCHENG。ERXINJIXIBAODEJIEGOUSHENGZHANG、GONGNENGCHENGSHUYIJIBINGTAIFAZHANJUNYUXIBAODENENGLIANGSHEQUHEDAIXIENENGLIXIANGGUAN,BIAOXIANCHUXIBAOYANGHUAHAIYUANPINGHENGZHUANGTAIDECHAYI。YINCI,JIZHIYINGDUSHIDIAOKONGXINJIXIBAOSHENGLI、BINGLIGUOCHENGDEZHONGYAOYINSU,YANJIUXINJIXIBAODEYANGHUAHAIYUANZHUANGTAIYUQIJIZHIYINGDUZHIJIANDEGUANXIYOUZHUYUSHENRULIJIEXINJIXIBAODESHENGLIHEBINGLIJILI。

西安交通大学仿生工程与力学研究所(BEBC)的研究人员首先以不同硬度聚丙烯酰胺水凝胶为基底,构建了体外心肌细胞的力学微环境模型。之后应用在细胞原位表征方面具有优势的扫描电化学显微镜(scanning electrochemical microscopy,SECM),以二茂铁甲酸(FcCOOH)为氧化还原电对,对不同硬度基质上培养的心肌细胞表面释放的重要的氧化还原态物质—谷胱甘肽(GSH)—与FcCOOH的氧化还原反应动力学常数(kf)进行了原位表征,得到了不同硬度基底上心肌细胞的氧化还原状态参数。结果发现,生长在越硬基底上的心肌细胞的kf值越高,表明心肌细胞较高的GSH释放速率。另外,不同基底硬度对心肌细胞氧化还原水平(kf值)的影响在培养第三天出现显著差异,之后随着培养时间的延长,心肌细胞的kf值趋于稳定。该结果表明心肌细胞所处的力学微环境对其氧化还原水平具有显著的调控作用。该工作也是**将扫描电化学显微镜用于细胞力学微环境的研究中,为探究细胞微环境对细胞氧化还原状态的影响提供了单细胞研究SECM新手段。


GAICHENGGUOYI“Effect of substrate stiffness on redox state of single cardiomyocyte: a scanning electrochemical microscopy study”WEITIFABIAOZAIAnalytical ChemistrySHANG。BOSHISHENGLIYABEIWEIDI1ZUOZHE,LIFEIJIAOSHOUWEIGAIWENDETONGXUNZUOZHE。GAIGONGZUODEDAOJIANADAXIANDALVEDAXUEDINGZHIFENGJIAOSHOUHEMEIGUOHAFOYIQIGONGSIFrank WangBOSHIZAILILUNMONIHEJISHUFANGMIANDEBANGZHU,YIJIXUFENGJIAOSHOUJIBEBCLAOSHIHETONGXUEMENDEDALIXIEZHU。GAIYANJIUDEDAOGUOJIAZIRANKEXUEJIJIN、ZHONGYANGGAOXIAOJIBENYEWUFEIDENGXIANGMUDEZIZHU。

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